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Morphological changes in the subpodocyte space (SPS) revealed by SE-SEM: comparison between perfused and non-perfused samples. Segmentation of the SPS area in images acquired from <t>BTBR</t> <t>WT</t> mice, perfused ( A , B ) and non-perfused ( C , D ), and from BTBR ob/ob mice, perfused ( E , F ) and non-perfused ( G , H ). SPS is highlighted in yellow. Scale bars: 10 μm (A, C, E, G), 5 μm (B, D, F, H).
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Morphological changes in the subpodocyte space (SPS) revealed by SE-SEM: comparison between perfused and non-perfused samples. Segmentation of the SPS area in images acquired from BTBR WT mice, perfused ( A , B ) and non-perfused ( C , D ), and from BTBR ob/ob mice, perfused ( E , F ) and non-perfused ( G , H ). SPS is highlighted in yellow. Scale bars: 10 μm (A, C, E, G), 5 μm (B, D, F, H).

Journal: Scientific Reports

Article Title: A scanning electron microscopy—based approach to explore subpodocyte space remodeling in diabetic kidneys of mice and humans

doi: 10.1038/s41598-026-40816-9

Figure Lengend Snippet: Morphological changes in the subpodocyte space (SPS) revealed by SE-SEM: comparison between perfused and non-perfused samples. Segmentation of the SPS area in images acquired from BTBR WT mice, perfused ( A , B ) and non-perfused ( C , D ), and from BTBR ob/ob mice, perfused ( E , F ) and non-perfused ( G , H ). SPS is highlighted in yellow. Scale bars: 10 μm (A, C, E, G), 5 μm (B, D, F, H).

Article Snippet: This study was performed on kidney samples from male BTBR ob/ob and BTBR WT mice obtained from Jackson Laboratories (Bar Harbor, ME).

Techniques: Comparison

Quantification of morphological changes in the subpodocyte space (SPS) in perfused and non-perfused samples. Quantification of SPS total area ( A ); percentage of tuft occupied by SPS ( B ), mean SPS area per podocyte ( C ), mean glomerular area ( D ) and podocyte density ( E ), in BTBR WT and BTBR ob/ob mice, perfused and non-perfused ( n = 2 animals per group, 5 glomeruli per animal). ** P < 0.0001, * P < 0.05. Data are presented as mean ± SEM.

Journal: Scientific Reports

Article Title: A scanning electron microscopy—based approach to explore subpodocyte space remodeling in diabetic kidneys of mice and humans

doi: 10.1038/s41598-026-40816-9

Figure Lengend Snippet: Quantification of morphological changes in the subpodocyte space (SPS) in perfused and non-perfused samples. Quantification of SPS total area ( A ); percentage of tuft occupied by SPS ( B ), mean SPS area per podocyte ( C ), mean glomerular area ( D ) and podocyte density ( E ), in BTBR WT and BTBR ob/ob mice, perfused and non-perfused ( n = 2 animals per group, 5 glomeruli per animal). ** P < 0.0001, * P < 0.05. Data are presented as mean ± SEM.

Article Snippet: This study was performed on kidney samples from male BTBR ob/ob and BTBR WT mice obtained from Jackson Laboratories (Bar Harbor, ME).

Techniques: